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KMID : 0861020130280040033
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Korea Journal of Herbology
2013 Volume.28 No. 4 p.33 ~ p.40
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Apoptosis Induction of HCT-15 Cells by Extracts of Undaria pinnatifida with Fermented Micro-organism
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김태윤:Kim Tae-Yun
한효상:Han Hyo-Sang/이영종:Lee Young-Jong
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Abstract
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Objectives : To study the apoptosis effects of fermented Undaria pinnatifida extracts(FUP) against HCT-15
colon cancer cells.
Method : By measuring cell proliferation, DNA fragmentation, cell cycle, morphology, and western blot from
FUP, the study investigated the effects of the extractions had upon the HCT-15 colon cancer cells, and
concluded that the inhibiting effects upon cells were induced by apoptosis.
Result : FUP effectively inhibited the growth of HCT-15 colon cancer cells. After analyzing the DNA
fragmentation, the study observed a DNA ladder, while examining the cells, and found an increase of sub-G1
hypodiploid cells. On the changes regarding the nucleus of the cells, a condensation of cells and chromatin, as
well as an apoptotic body was clearly observed. By observing through western blot from FUP, the study found
a decreased level of Bcl-2 from HCT-15 colon cancer cells, but the increased level of Bax and cleaved
caspase-3, which as a result induced apoptosis, inhibiting the growth of HCT-15 colon cancer cells. FUP
increased the natural death of HCT-15 colon cancer cells by the induction of apoptosis. FUP seemed to have
no suppressing effect upon HL-60/MX2 cells. However, compared to the fucoidan, the study was able to clearly
observe morphological changes of HCT-15 cells apoptosis, in a 1/2 concentration.
Conclusion : FUP had antiproliferative effects on different kinds of cancer cells, while proving especially
efficacious against colon cancer cells.
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KEYWORD
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fermented Undaria pinnatifida, colon cancer cell, apoptosis
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